Journal: Molecular Medicine Reports

Article Title: Platelet-derived growth factor D promotes the angiogenic capacity of endothelial progenitor cells

doi: 10.3892/mmr.2018.9692

Figure Lengend Snippet: PDGF-D functions via PDGFR-dependent and -independent pathways. (A-E) Western blot analysis was performed on the total protein extracted from confluent PDGF-D-EPCs and GFP-EPCs. (1–1, 1–2, 1–3 stand for GFP-EPCs and 2–1, 2–2, 3–3 represent PDGF-D-EPCs). The expression and phosphorylation of STAT3, AKT, ERK1/2, mTOR and GSK-3β were measured by immunoblotting, and normalized to the levels of GAPDH. **P<0.01 vs. the control group. (F) The expression of VEGF, HGF and PDGF-B was examined by RT-qPCR. The expression levels were normalized to GAPDH; **P<0.01 vs. the control group.

Article Snippet: The following antibodies (Abs) (Cell Signaling Technology, Inc., Danvers, MA, USA) were used (all dilutions, 1:1,000): GSK-3β Ab (cat no. 9315), phospho-GSK-3β Ab (cat no. 9323), STAT3 Ab (cat no. 8232), phospho-STAT3 Ab (cat no. 9134), mTOR Ab (cat no. 2972), phospho-mTOR Ab (cat no. 2971), ERK1/2 Ab (cat no. 4696), phospho-ERK1/2 Ab (cat no. 8544) and PDGF-D Ab (SCBT, Dallas, TX, USA).

Techniques: Western Blot, Expressing, Phospho-proteomics, Control, Quantitative RT-PCR

Journal: International Journal of Oncology

Article Title: NQO1 drives glioblastoma cell aggressiveness through EMT induction via the PI3K/Akt/mTOR/Snail pathway

doi: 10.3892/ijo.2023.5558

Figure Lengend Snippet: NQO1 regulates GBM cell proliferation in vitro and in vivo . (A) Expression levels of NQO1 in NHA and GBM cell lines were detected by western blotting. (B) Confirmation of NQO1 knockdown and overexpression were detected by western blotting in the sh-Con, sh-NQO1, vector and NQO1 overexpression groups. β-actin was used as a loading control. Effects of NQO1 on GBM cell proliferation were determined using (C) MTT, (D) EdU and (E) colony formation assays. Scale bar: 50 μ m. (F) Expression levels of cell cyclin-related proteins in the NQO1 knockdown or overexpression groups were detected by western blotting. (a) sh-Con group, (b) shNQO1 group, (c) vector group (d) NQO1 overexpression group. (G) Representative images of GBM cell xenograft tumors in the four groups of nude mic. . (a) sh-Con group, (b) shNQO1 group, (c) vector group (d) NQO1 overexpression group. Xenograft tumor weights are shown (n=4/group). In the images, each grid represents 1×1 mm. (H) Ki67 expression in tumor sections were determined by immunohistochemical analysis. Scale bar: 50 μ m. In all panels, t-tests for independent means were used for two group comparisons. * P<0.05, ** P<0.01. NQO1, NAD(P)H:quinone acceptor oxidoreductase 1; NHA, normal human astrocytes; GBM, glioblastoma multiforme; sh, short hairpin; Con, control; CDK1, cyclin-dependent kinase 1.

Article Snippet: Antibodies against Slug (cat. no. #9585), twist-related protein (Twist; cat. no. #90445), p27 (cat. no. #3686), Cyclin B (cat. no. #4138), Cyclin D (cat. no. #55506), cyclin-dependent kinase 1 (CDK1; cat. no. #77055), eIF4E-binding protein (4EBP1; cat. no. #9644), phosphorylated (p)-4EBP1 (cat. no. #2855), p-ribosomal protein S6 (S6; cat. no. #4858), S6 (cat. no. #2317), p-Akt (cat. no. #4060), Akt (cat. no. #9272), p-PI3K (cat. no. #17366), PI3K (cat. no. #4255), p-mammalian target of rapamycin (mTOR; cat. no. #2971), mTOR (cat. no. #2972) and β-actin (cat. no. #93473) were purchased from Cell Signaling Technology, Inc. Antibodies against Ki67 (cat. no. 27309) and Snail (cat. no. 13099) were purchased from Proteintech Group, Inc.

Techniques: In Vitro, In Vivo, Expressing, Western Blot, Knockdown, Over Expression, Plasmid Preparation, Control, Immunohistochemical staining